dynamic BIOSENSORS AS-2-Gc-lfs Adapter Strand 2 User Manual

Fa'afefiloi strand Adapter 1 – Gc (400 nM) ma le laina Ligand conjugated (500 nM) ile 1:1 ratio (v/v).
Fa'asusu le vaifofo mai le Laasaga 1 i le vevela o le potu ile 600 rpm mo le 30 minute mo le fa'afefiloi atoatoa.

Faafefiloi le vaifofo mai le Laasaga 2 ma le Adapter strand 2 – Gc – lfs (200 nM) i le fua faatatau 1:1 (v/v).
Ua sauni nei le fofo mo le fa'atinoina o le biochip.
Manatua: O le mautu o le fofo e faʻalagolago i le mautu o le mole mole ligand.

FAQ

O le a le umi e teu ai le Adapter strand 2 – Gc-lfs?
O le oloa e fa'atapula'aina le ola. Fa'amolemole va'ai i le aso e muta ai le aoga i luga o le fa'ailoga mo fa'amatalaga patino.

E mafai ona fa'aoga le Adapter strand 2 – Gc-lfs mo fa'amoemoega e ese mai i su'esu'ega?
Leai, o lenei oloa ua fa'amoemoe mo na'o su'esu'ega.

Vaega Autu

Feso'ota'i strand 2 mo le fa'atinoina o le heliX® Adapter Chip Spot 2.

E fetaui ma le heliX® Adapter Chip.
E aofia ai fa'amau Feso'ota'i mo 50 toe fa'afouina.
Lelei mo MIX&RUN sample sauniuniga.
Adapter strand 2 o lo'o ave ai se vali lanu meamata hydrophobic (Gc) fa'atasi ai ma se tau fa'amautu.

HeliX® Adapter Chip Overview

2 nofoaga faʻatasi ma 2 taula eseese faʻasologa mo le DNA-encoded tuatusi.

Fa'amatalaga o oloa

Numera o le Poloaiga: AS-2-Gc-lfs
Laulau 1. Anotusi ma Fa'amatalaga Teuina

Mea	Cap	Fa'atotonuga	Aofaiga	Puipui	Teuga
*Fa'amau fa'amau 2 – Gc-lfs*	Lanu pa'epa'e	200/250 nM	5 x 200 µL	TE40 [1]	-20°C

po'o su'esu'ega fa'aoga. O lenei oloa e fa'atapula'aina le ola, fa'amolemole va'ai le aso e muta ai le fa'ailoga. Ina ia aloese mai le tele o ta'amilosaga fa'a'aisa fa'amolemole fa'aliquot le nanolever.

Sauniuniga | MIX&RUN

Fa'ato'a fa'atosinaina o mea fa'apipi'i ma manoa ligand:

Fa'afefiloi strand Adapter 1 – Gc (400 nM) ma le laina Ligand conjugated (500 nM) ile 1:1 ratio (v/v).

Fa'asusu le vaifofo o le Laasaga 1 ile RT ile 600 rpm mo le 30 min e fa'amautinoa ai le fa'ato'a atoatoa.
Faafefiloi le vaifofo o le Laasaga 2 ma le Adapter strand 2 – Gc – lfs (200 nM) ile 1:1 ratio (v/v).

Ua sauni le fofo e fa'aoga mo le fa'atinoina o le biochip. O le mautu o le fofo e fesoʻotaʻi ma le mautu o le mole mole ligand.

Laulau 2. Mea fa'aopoopo mo le fa'atinoina o le vaega 1 ma le fa'asinomaga 2.

Mea	Fa'atotonuga	Puipui	Igoa Oloa Fa'atatau	Poloaiga Nu
*Fa'asu'ega strand 1 – Gc*	400 nM	TE40 [1]	*Fa'asuesu'e filifili 1* ma le vali lanu meamata Gc	AS-1-Gc
*Ligand* *manoa* amoina le ligand conjugated	500 nM	P2] E40 [	helikopaX® Amine Coupling Kit 1	HK-NHS-1

Example
Volume mana'omia mo le 3 fa'atinoga: 100 µL fa'atasi ai ma le fa'ai'uga mulimuli o le 100 nM.

Vial 1		Vial 2
*Fa'asu'ega strand 1 – Gc* (400 nM)	Fa'atasi *Ligani manoa* (500 nM)	*Fa'amau su'esu'e 2 – Gc – lfs* (200/250 nM)
25 µL	25 µL	50 µL

A maeʻa le taimi faʻafefe, faʻafefiloi le vial 1 ma le vial 2 e maua ai le 100 μL o le DNA ua saunia e faʻaaoga.

Fa'afeso'ota'i

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©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Ua taofia aia tatau uma.[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA [2] Afai e le mautu le polotini i le PE40 (TE40, HE40), faamolemole siaki le fetaui ma le switchSENSE® pepa fetaui.

Pepa / Punaoa

dynamic BIOSENSORS AS-2-Gc-lfs Adapter Strand 2 [pdf] Tusi Taiala
AS-2-Gc-lfs v5.1, AS-2-Gc-lfs Feso'ota'i Fusi 2, AS-2-Gc-lfs, Feso'ota'i Fa'amau 2, Fa'amau 2

Fa'asinomaga

Tusi Taiala

dynamic BIOSENSORS AS-2-Gc-lfs Adapter Strand 2

Fa'amatalaga

Sauniuniga ma Fa'atonuga Fa'aaogā

FAQ