malosi BIOSENSORS heliX fa'atasi ai ma Adapter Strand 2
Vaega Autu
- Feso'ota'i strand 2 mo le fa'atinoina o le heliX® Adapter Chip Spot 2.
- E fetaui ma le heliX® Adapter Chip.
- E aofia ai fa'amau Feso'ota'i mo 50 toe fa'afouina.
- Lelei mo MIX&RUN sample sauniuniga.
- Adapter strand 2 o lo'o feavea'i se vali mumu hydrophobic (Rc) fa'atasi ai ma se tau fa'aletonu.
HeliX® Adapter Chip Overview
2 nofoaga faʻatasi ma 2 taula eseese faʻasologa mo le DNA-encoded tuatusi.
Fa'amatalaga o oloa
Numera o le Poloaiga: AS-2-Rc-lfs
Laulau 1. Anotusi ma Fa'amatalaga Teuina
| Mea | Cap | Fa'atotonuga | Aofaiga | Puipui | Teuga |
| Fa'amau fa'amau 2 – Rc – lfs | Lanu pa'epa'e | 200/250 nM | 5 x 200 µL | TE40 [1] | -20°C |
Mo na'o su'esu'ega fa'aoga.
O lenei oloa e fa'atapula'a lona ola, fa'amolemole va'ai le aso e muta ai le fa'ailoga.
Ina ia aloese mai le tele o ta'amilosaga fa'a'aisa fa'amolemole fa'aliquot le nanolever.
Sauniuniga | MIX&RUN
Fa'ato'a fa'atosinaina o mea fa'apipi'i ma manoa ligand:
- Fa'afefiloi strand Adapter 1 – Rc (400 nM) ma le laina Ligand conjugated (500 nM) ile 1:1 ratio (v/v).
- Fa'asusu le vaifofo o le Laasaga 1 ile RT ile 600 rpm mo le 30 min e fa'amautinoa ai le fa'ato'a atoatoa.
- Faafefiloi le vaifofo o le Laasaga 2 ma le Adapter strand 2 – Rc – lfs (200 nM) i le 1:1 ratio (v/v).
Ole fofo ua sauni e fa'aoga mo le fa'atinoina ole biochip.
O le mautu o le fofo e fesoʻotaʻi ma le mautu o le mole mole ligand.
Laulau 2. Mea fa'aopoopo mo le fa'atinoina o le vaega 1 ma le fa'asinomaga 2.
| Mea | Fa'atotonuga | Puipui | Igoa Oloa Fa'atatau | Poloaiga Nu |
| Fa'amau fa'asuesu'e 1 – Rc | 400 nM | TE40 [1] | Fa'asuesu'e filifili 1 ma le vali mumu Rc | AS-1-Rc |
| Ligand manoa
amoina le ligand conjugated |
500 nM | 2P] E40 [ | HeliX® Amine Coupling Kit 1 | HK-NHS-1 |
Example
Volume manaʻomia mo 3 faʻatinoga: 100 μL faʻatasi ai ma le faʻatonuga mulimuli o le 100 nM.
| Vial 1 | Vial 2 | |
| Fa'amau fa'asuesu'e 1 – Rc
(400 nM) |
Fa'atasi Ligani manoa
(500 nM) |
Fa'amau fa'amau 1 – Rc – lfs
(200/250 nM) |
| 25 µL | 25 µL | 50 µL |
A maeʻa le taimi faʻafefe, faʻafefiloi le vial 1 ma le vial 2 e maua ai le 100 μL o le DNA ua saunia e faʻaaoga.
Fa'afeso'ota'i
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Siamani
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
ISA
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Lagolago Fa'atekinisi support@dynamic-biosensors.com
www.dynamic-biosensors.com
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©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Ua taofia aia tatau uma.
- TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
- Afai e le mautu le polotini i le PE40 (TE40, HE40), fa'amolemole siaki le fetaui lelei ma le laupepa feso'ota'iga switchSENSE®.
Pepa / Punaoa
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malosi BIOSENSORS heliX fa'atasi ai ma Adapter Strand 2 [pdf] Tusi Taiala AS-2-Rc-lfs v5.1, heliX fa'aopoopo Feso'ota'i Fa'amau 2, Feso'ota'i Fa'amau 2, Fa'amau 2 |
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malosi BIOSENSORS heliX fa'atasi ai ma Adapter Strand 2 [pdf] Tusi Taiala heliX fa'aopoopo Feso'ota'iga Strand 2, heliX fa'aopoopo, Feso'ota'i Strand 2, Strand 2 |